RPS6KB1 (T412E) (Human) Recombinant Protein
产品名称： RPS6KB1 (T412E) (Human) Recombinant Protein
英文名称： RPS6KB1 (T412E) (Human) Recombinant Protein
产品编号： P5755
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Human RPS6KB1 (NP_003152.1, 1 a.a. - 421 a.a.) T412E mutant partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 75
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 91 % by SDS-PAGE/CBB staining
- Activity:
- The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate : S6K2 peptide. ATP: 100 µM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- SDS-PAGE Stained with Coomassie Blue
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 6198
- Protein Accession#:
- NP_003152.1
- Gene Name:
- RPS6KB1
- Gene Alias:
- PS6K,S6K,S6K1,STK14A,p70(S6K)-alpha,p70-S6K,p70-alpha
- Gene Description:
- ribosomal protein S6 kinase, 70kDa, polypeptide 1
- Omim ID:
- 608938
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq
- Other Designations:
- p70 S6 kinase, alpha 1,p70 S6 kinase, alpha 2,ribosomal protein S6 kinase, 70kD, polypeptide 1,serine/threonine kinase 14 alpha